A rapid and simple yeast transformation procedure has been developed using colonies on agar plates. Saccharomyces cerevisiae SHY3 cells were picked up from colonies on YPD plates grown freshly or stored at 4 degrees C and incubated with M13RK9-T DNA at 30 degrees C for 1-2 h in a solution of Li+, Ca
โฆ LIBER โฆ
A novel method for subcellular fractionation ofSaccharomyces cerevisiae
โ Scribed by Kevin J. Blackwell; Jane S. White; John M. Tobin
- Book ID
- 110270940
- Publisher
- Springer-Verlag
- Year
- 1999
- Tongue
- English
- Weight
- 45 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0951-208X
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