A gene tightly linked toCEN6is important for growth ofSaccharomyces cerevisiae

✍ Scribed by Maria Luisa Agostoni Carbone; Manuela Solinas; Silvio Sora; Lucia Panzeri

• Book ID: 104739610
• Publisher: Springer-Verlag
• Year: 1991
• Tongue: English
• Weight: 803 KB
• Volume: 19
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00362080

✦ Synopsis

Transcriptional analysis of the region flanking the left boundary of the centromere of chromosome VI revealed the presence of a gene immediately adjacent to CENt. The transcription of the gene is directed toward the centromere, and nucleotide sequence analysis showed that the coding region terminates only 50 bp away from CEN6. Our results extend to chromosome VI the observation that centromere-flanking regions of S. cerevisiae are transcriptionally active. Disruption of the coding region of the gene showed that its product, whilst not essential for cell viability, is important for normal cell growth. The gene has been termed DEG1 (DEpressed Growth rate). Comparison of the deduced amino acid sequence of DEGI with a protein sequence databank revealed homology with the enzyme tRNA pseudouridine synthase I of E. coli.