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A novel method for purification of inner cell mass and trophectoderm cells from blastocysts using magnetic activated cell sorting

✍ Scribed by Manabu Ozawa; Peter J. Hansen


Book ID
116478205
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
318 KB
Volume
95
Category
Article
ISSN
1556-5653

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A rapid procedure has been devised to count the numbers of outer trophectoderm (TE) and inner cell mass (ICM) cells of mouse blastocysts by differentially labelling their nuclei in situ with polynucleotide-specific fluorochromes. The TE nuclei were labelled with propidium iodide (PI) by permeabilisi