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A novel method for cryopreservation of individual human spermatozoa

✍ Scribed by Qiu-Ping Peng; Shao-Feng Cao; Qi-Feng Lyu; Song-Guo Xue; Wei Jin; Xiao-Yin Liu; Wen-Jie Zhang; H. Ingolf Nielsen; Yan-Ping Kuang


Book ID
107427373
Publisher
Society for In Vitro Biology
Year
2011
Tongue
English
Weight
209 KB
Volume
47
Category
Article
ISSN
1475-2689

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✍ Dr. Franck Pellestor; Anne Girardet; GeneviΓ¨ve Lefort; Brigitte AndrΓ©o; Jean Pau πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons 🌐 English βš– 512 KB

## Abstract Direct in situ labeling of human spermatozoa was performed using the PRINS method. This technique is based on annealing of specific oligonucleotide primers, and subsequent primer extension by a __Taq__ DNA polymerase. The reaction was carried out on a programmable temperature cycler, an