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A Novel Genetic Selection System for Improved Enantioselectivity of Bacillus subtilis Lipase A

✍ Scribed by Ykelien L. Boersma; Melloney J. Dröge; Almer M. van der Sloot; Tjaard Pijning; Robbert H. Cool; Bauke W. Dijkstra; Wim J. Quax

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
249 KB
Volume
9
Category
Article
ISSN
1439-4227

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## Abstract This article describes a simple and robust laboratory exercise on the regulation of membrane unsaturated fatty acid composition in bacteria by a decrease in growth temperature. We take advantage of the well characterized Des pathway of __Bacillus subtilis__, composed of a Δ5‐desaturase