✦ LIBER ✦

Recombinant Bacillus subtilis whole cell system as a catalyst for enzymatic synthesis of cyclic inositol phosphate

✍ Scribed by Anil K Tyagi; Ram A Vishwakarma

Publisher: Elsevier Science
Year: 1998
Tongue: French
Weight: 235 KB
Volume: 39
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(98)01250-7

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✦ Synopsis

Whole cell culture of recombinant bacteria Bacillus subtilis, over expressing the phosphoinositide specific phospholipase C (PI-PLC) gene of Bacillus thuringiensis has been used as an efficient catalyst for practical muhigram scale synthesis of D-myo-inositol 1,2-cyclic monophosphate directly from phospholipid mixtures containing phosphatidyi-inositol. This synthesis does not require protein purification and growing bacterial culture can be directly used. The reaction can be monitored by in-situ 31p NMR.

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