A novel chemiluminescent method for determination of phloroglucinol

✍ Scribed by Hua Cui; Cheng-gen Xie; Chun-ze Lai

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 135 KB
• Volume: 18
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.740

✦ Synopsis

It was found that the inhibition and enhancement by phloroglucinol of the chemiluminescence from the luminol-K 3 Fe(CN) 6 system were dependent on the pH of luminol solution and the concentration of phloroglucinol. In Na 2 CO 3 -NaHCO 3 buffer, phloroglucinol exhibited strong chemiluminescent enhancement at pH 9.4. On this basis, a flow injection method was developed for the determination of phloroglucinol. The method was simple, rapid, convenient and sensitive, with a detection limit of 2.0 × 10 -9 mol/L. It is effective for determining phloroglucinol in the range of 1.0 × 10 -5 -5.0 × 10 -9 mol/L. The relative standard deviation is 1.3% within one day and 3.2% between days for the determination of 5.0 × 10 -7 mol/L phloroglucinol. The method has been successfully used to determine phloroglucinol in environmental water, with satisfactory results.