An efficient method of growing the protozoon Tetrahymena to high cell densities in a 2-1 bioreactor is described. The first phase of the fermentation is a batch phase with minimum generation times (1.4 h). During the next phase medium is exchanged continuously using a perfusion module based on micro
A Novel Bioreactor-Cell Precipitator Combination for High-Cell Density, High-Flow Fermentations
β Scribed by Gregory Stephanopoulos; K.-Y. San; B. H. Davison
- Book ID
- 118282531
- Publisher
- American Institute of Chemical Engineers
- Year
- 1985
- Tongue
- English
- Weight
- 993 KB
- Volume
- 1
- Category
- Article
- ISSN
- 8756-7938
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VERO cells were cultivated in microcarriers (MCs) using a bioreactor with a working capacity of 3.7 1. We studied the effect of MCs concentration in the proportion of MC-bearing cells and on the kinetics of cell growth, as well as the cell growth with a batch, fed-batch and perfusion mode operation.