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A Novel Approach to DNA Damage Assessments: Measurement of the Thymine Glycol Lesion

✍ Scribed by Douglas T. Bailey, Han-Chun C. DeFedericis, Kellee F. Greene, Herbert Iijima, Edwin E. Budzinski, Helen B. Patrzyc, Jean B. Dawidzik and Harold C. Box

Book ID
124650812
Publisher
Bioone
Year
2006
Tongue
English
Weight
952 KB
Volume
165
Category
Article
ISSN
0033-7587

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Gas chromatographic-mass spectrometric m
Gas chromatographic-mass spectrometric method for the assessment of oxidative damage to double-stranded DNA by quantification of thymine glycol residues
✍ S.P. Markey; C.J. Markey; Wang T.-C.L.; RodrΓ­guez J.B. πŸ“‚ Article πŸ“… 1993 πŸ› Elsevier Science 🌐 English βš– 750 KB

A technique for the measurement of thymine glycol at parts per million concentrations in double-stranded polymeric DNA is described. The procedure utilizes base to ring-open DNA-bound thymine glycol in the presence of monomeric [(2)H4]thymine glycol as an internal standard, followed by reduction, so