✦ LIBER ✦

A nonlinear wide-range immobilized pH gradient for two-dimensional electrophoresis and its definition in a relevant pH scale

✍ Scribed by Bengt Bjellqvist; Christian Pasquali; Florence Ravier; Jean-Charles Sanchez; Dr. Denis Hochstrasser

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 901 KB
Volume: 14
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.11501401209

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Improved method for identification of pr

Improved method for identification of proteins using two-dimensional electrophoresis with immobilized pH gradient isoelectric focusing

✍ Dr. Robert B. Mackin; Veronica A. Raker; Joachim Spiess 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 360 KB 👁 1 views

Recent advances in protein sequence analysis now permit the determination of partial N-terminal and internal primary structure from low picomole quantities of protein. The major remaining hurdles to sequence analysis of small amounts of protein are the identification, isolation, and handling of micr

Two-dimensional electrophoretic separati

Two-dimensional electrophoretic separation of yeast proteins using a non-linear wide range (pH 3–10) immobilized pH gradient in the first dimension; reproducibility and evidence for isoelectric focusing of alkaline (pI >7) proteins

✍ Norbeck, Joakim; Blomberg, Anders 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 346 KB 👁 2 views

The proteome of the yeast Saccharomyces cerevisiae was analysed by two-dimensional (2D) polyacrylamide gel electrophoresis utilizing a non-linear immobilized pH gradient (3-10) in the first-dimensional separation. Cells were labelled by [ 35 S]methionine incorporation in the respiro-fermentative pha

Serum proteins of rats exposed to organi

Serum proteins of rats exposed to organic solvents examined by horizontal two-dimensional electrophoresis with an immobilized pH gradient in the first dimension

✍ Gabriela Zastrow; Dr.-Ing Siegfried Günther; Angelika Görg; Wilhelm Postel; Hors 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 362 KB 👁 2 views

Kat serum IPG-Dalt after solvent inhalation 655 strate these differences the staining reaction shown in Fig. was intentionally extended, with resultant impaired resolution of strongly reacting isoforms from A. nodosum (lane 1). Additionally, Fig. demonstrates a positive reaction of all isoenzymes