Serum proteins of rats exposed to organic solvents examined by horizontal two-dimensional electrophoresis with an immobilized pH gradient in the first dimension

✍ Scribed by Gabriela Zastrow; Dr.-Ing Siegfried Günther; Angelika Görg; Wilhelm Postel; Horst A. Diehl; Eugéne H. J. M. Jansen

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 362 KB
• Volume: 11
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150110815

✦ Synopsis

Kat serum IPG-Dalt after solvent inhalation 655 strate these differences the staining reaction shown in Fig. was intentionally extended, with resultant impaired resolution of strongly reacting isoforms from A. nodosum (lane 1). Additionally, Fig. demonstrates a positive reaction of all isoenzymes from A . nodosum with anti-A.n.1 antibodies (IB, lane 1) whereas only two isoforms from L. digitata exhibited weak immunological cross-reactivity ( lB, lanes 2-5). This comparison also required an extended staining reaction. The above results demonstrate that DEAE-cellulose is useful for protein blotting, affording good resolution and high binding capacities for proteins 13,4, 151.