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A new specific DNA endonuclease activity in yeast mitochondria

โœ Scribed by Sargueil, B. ;Delahodde, A. ;Hatat, D. ;Tian, G. L. ;Lazowska, J. ;Jacg, C.


Book ID
104694489
Publisher
Springer
Year
1991
Tongue
English
Weight
168 KB
Volume
225
Category
Article
ISSN
0026-8925

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โœฆ Synopsis


Two group I intron-encoded proteins from the yeast mitochondrial genome have already been shown to have a specific DNA endonuclease activity. This activity mediates intron insertion by cleaving the DNA sequence corresponding to the splice junction of an intronless strain. We have discovered in mitochondrial extracts from the yeast strain 777-3A a new DNA endonuclease activity which cleaves the fused exon A3-exon A4 junction sequence of the CO XI gene.


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A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described. DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligon