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A new, sensitive ecto-5′-nucleotidase assay for compound screening

✍ Scribed by Freundlieb, Marianne; Zimmermann, Herbert; Müller, Christa E.

Book ID
122139264
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
721 KB
Volume
446
Category
Article
ISSN
0003-2697

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✍ Jörg Servos; Helmut Reiländer; Herbert Zimmermann 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 172 KB

To establish a procedure for reproducible production of a soluble enzymatically active form of rat ecto-5′-nucleotidase, we heterologously expressed the polypeptide of the mature protein in insect cells. An expression construct was created consisting of this polypeptide and glutathione-S-transferase