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A new, rapid and sensitive bioluminescence assay for drug screening on Leishmania

✍ Scribed by Paloque, Lucie; Vidal, Nicolas; Casanova, Magali; Dumètre, Aurélien; Verhaeghe, Pierre; Parzy, Daniel; Azas, Nadine

Book ID
121767683
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
458 KB
Volume
95
Category
Article
ISSN
0167-7012

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A new, simple, and very sensitive assay for phospholipase A and C is described. The assay is based on the bioluminescence developed by the mutant of the bacterium Beneckea harveyi as a response to myristic acid released from dimyristoyl phosphatidylcholine by either phospholipase A or by a phospholi