A new, fast, and very sensitive bioluminescence assay for phospholipases A and C

✍ Scribed by Shimon Ulitzur; Michael Heller

• Book ID: 102982887
• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 571 KB
• Volume: 91
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90527-4

✦ Synopsis

A new, simple, and very sensitive assay for phospholipase A and C is described. The assay is based on the bioluminescence developed by the mutant of the bacterium Beneckea harveyi as a response to myristic acid released from dimyristoyl phosphatidylcholine by either phospholipase A or by a phospholipase C-lipase coupled system. It is possible to assay these enzymes at a rate corresponding to a release of as little as 1 to 2 pmol of myristic acid per minute.