A simple and sensitive fluorometric assay was developed to test renin activity within several hours. Two new fluorogenic peptides, Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide (octapeptide-MCA) and a succinyl derivative of the octapeptide-MCA were synthesized and used as a renin substrat
A new radiolabeled protein renin substrate
✍ Scribed by Kenneth E. Lentz; Leonard T. Skeggs; Frederic E. Dorer; Joseph R. Kahn; Melvin Levine
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 692 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0003-2697
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## Abstract Boc‐hydrazine (1) was treated with 4‐nitrophenyl chloroformate (2) to give Boc‐Azagly 4‐nitrophenyl ester (3). Reaction of 3 with H‐ACHP‐Ile‐3‐pyridylmethylamide^5)^ (4) provided Boc‐Azagly‐ACHP‐Ile‐3‐pyridylmethylamide (5). This was deprotected with HCl/dioxane to give H‐Azagly‐ACHP‐Il