A new protected acyl protecting group for exocyclic amino functions of nucleobases

✍ Scribed by C.M. Dreef-Tromp; P. Hoogerhout; G.A. van der Marel; J.H. van Boom

• Book ID: 104226626
• Publisher: Elsevier Science
• Year: 1990
• Tongue: French
• Weight: 276 KB
• Volume: 31
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(00)94573-8

✦ Synopsis

2-(Terf-butytdiphenylsiiybxymethyl)benzoyl chbrkfe (SiOMB-Cl) reads with the per-0-trimethylsilylated dnucleoskbs C,G and A to give, after removal of the Si(Me), groups and 5'-O-pmtectbn with 4,4'dimethoxytrityl chbride (DMT-Cl), the corresponding NSiOMB-5'-O-DMT derivates, the SiOMB groups of which can easily he removed hy fluoride ion. Ths SiOMB protected nudeoskfes proved to he suitable building units for the preparatbn of DNA fragments in solution and on a solid support. Nucboproteins are naturally occunfng bibpolymers' in which the 5'-terminal hydmxyl group of nucleic acids (DNA or RNA) is covalently linked through a phosphodiister bond with the hydroxyl groups of the L-amino acids serine, threonine and (or) tyrosine in proteins. The results so far ohtainedP in preparing nucleopeptids fragments [i.e., l(R=H)] containing