A new procedure for the purification of
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A. PlΓ‘; A Chueca; J LΓ³pez-GorgΓ©
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Article
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1981
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Springer
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English
β 495 KB
A new purification procedure for spinach leaf fructose-l,6-bisphosphatase is proposed, which includes the use of affinity chromatography on mercaptoethylamine-Sepharose. A homogeneous preparation of the enzyme can be obtained in 48 hr, with a specific activity of 67 U/rag and a yield of 23%. The met