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A new procedure for the preparation of GDP-[U-14C]mannose

✍ Scribed by William A. Braell; Michael A. Tyo; Sharon S. Krag; Phillips W. Robbins

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
264 KB
Volume
74
Category
Article
ISSN
0003-2697

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✦ Synopsis

This paper describes the synthesis of GDP-[U-Wlmannose from [W4C]sucrose using invertase and a crude yeast preparation supplemented with hexokinase. The radiochemical yield of GDP-[U-14C]mannose is 20-30%; 95% of the label in the isolated product is GDP-['*C]mannose.

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