## Abstract The Ξ± and Ξ² hydrogen exchange reactions catalyzed by pyridoxal have been applied to ten of the common amino acids to prepare Ξ±, Ξ² deuterated derivatives. The exchange and isolation procedure allows for numerous different samples to be processed in parallel. The deuteration at the Ξ± posi
A new procedure for the labeling of peptides and amino acids
β Scribed by Derek H.R. Barton; Taewoo Kwon; Dennis K. Taylor; Marmood Tajbakhsh
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 597 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0968-0896
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β¦ Synopsis
Several stable, water soluble cyclohexa-2,4-dien-1-ones have been prepared and their photolytic coupling reactions in aqueous solution at 28 degrees C with various amino acids and dipeptides investigated. This procedure represents a new and high yielding method for the labeling of the terminal amino functionality of peptides and amino acids.
π SIMILAR VOLUMES
Optically pure L-valine was labelled with tritium by the Wilzbach method. Only 4 of the activity of the radiochemically pure valine was found in D-valine. This figure was determined by reverse isotope dilution. The suitability of this method for valine was tested, wich good results, with DL-valine