✦ LIBER ✦

A new procedure for handling impervious biological specimens

✍ Scribed by V. A. Lindley

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 777 KB
Volume: 21
Category: Article
ISSN: 1059-910X
DOI: 10.1002/jemt.1070210411

No coin nor oath required. For personal study only.

✦ Synopsis

A new application of techniques for preparing impervious biological specimens for light microscopy (LM) and transmission electron microscopy (TEM) has been developed. Microwave irradiation was used to facilitate fixation. A priming technique was used to increase the bonding of the outer surface of the specimens with the resin. Priming the waxy or cuticular surface with 2-6040 (gamma-glycidoxypropyl trimethoxysilane) solved the problem of specimen "pull out" from the resin. Insect specimens with various types of cuticles (waxy or chitinous) and seeds were successfully studied ultrastructurally using this technique. o 1992 Wiley-Liss, Inc.

📜 SIMILAR VOLUMES

An improved automated procedure for the

An improved automated procedure for the determination of calcium in biological specimens

✍ Hillel J. Gitelman 📂 Article 📅 1967 🏛 Elsevier Science 🌐 English ⚖ 590 KB

Chloretone Water: A New Preservative of

Chloretone Water: A New Preservative of Biological Specimens

✍ Farwell, Oliver Atkins 📂 Article 📅 1919 🏛 Elsevier ⚖ 145 KB

A procedure for handling microorganisms

A procedure for handling microorganisms during preparation for electron microscopy

✍ Griffin, Margaret ;Bilello, Leonard ;Mapp, Paulette ;Witkus, Ruth 📂 Article 📅 1987 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 178 KB

A new disaggregation device for cytology

A new disaggregation device for cytology specimens

✍ Peter S. Oud; David J. Zahniser; Ditty J. Haag; Maria C. G. van Boekel; Huub G. 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 583 KB

A new means of disaggregating cytology specimens in suspension using an immersible rotor device is described. The new rotor is compared to an automated syringing apparatus using cervical samples. Similar results us-ing both devices are obtained for both normal and abnormal specimens.

A method for the determination of proges

A method for the determination of progesterone in biological specimens

✍ D.J. Watson; E.B. Romanoff; J. Kato; D.B. Bartosik 📂 Article 📅 1967 🏛 Elsevier Science 🌐 English ⚖ 739 KB

The analysis of progesterone in blood and tissues is at best a tedious process. If many samples must be analyzed at one time, the difficulty increases enormously. By taking advantage of recent developments in thin-layer chromatography (TLC) (l), we have developed a method for the analysis of progest

A new procedure for exact ring closure

✍ M. D. Di Benedetto; P. Lucibello; A. L. Sangiovanni-Vincentelli; K. Yamaguchi 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 256 KB 👁 2 views