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A method for the determination of progesterone in biological specimens

โœ Scribed by D.J. Watson; E.B. Romanoff; J. Kato; D.B. Bartosik

Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
739 KB
Volume
20
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

The analysis of progesterone in blood and tissues is at best a tedious process. If many samples must be analyzed at one time, the difficulty increases enormously. By taking advantage of recent developments in thin-layer chromatography (TLC) (l), we have developed a method for the analysis of progesterone in whole blood and tissues which is rapid, sensitive, reliable, and adapted for the routine analyses of between 10 t,o 40 samples at one time.

The method combines the isolation of pure progesterone (as demonstrated by radiochemical technique) with quantitation based on the formation of progesterone-3,20-bisdinitrophenplhydrazone (prog-DNPH ) and consists of the following steps: 'Fellow,

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