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A new photometric assay for blood alcohol

✍ Scribed by J. Kovář; J. Schneider; L. Skurský; H. Dubský

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
416 KB
Volume
137
Category
Article
ISSN
0003-2697

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✦ Synopsis

The proposed method for ethanol determination is based on the simultaneous oxidation of ethanol and reduction of nitrosodimethylaniline to a quinonediimine derivative in the presence of NAD and horse liver alcohol dehydrogenase. Quinonediimine formed in this reaction is coupled with salicylamide and the absorbance of the resulting blue indaniline dye is measured. The new method yields identical results when compared with the currently used methods for the determination of blood alcohol (i.e., with the conventional enzymatic method, Widmark's method, and gas chromatography). Its main advantage is high sensitivity, little consumption of both enzyme and coenzyme, and the measurement in the visible range of the spectrum.

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