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A new minimal synthetic medium for germ-tube production inCandida albicans

✍ Scribed by Massimo Bruatto; Marilena Gremmi; Valerio Vidotto

Book ID
104772892
Publisher
Springer Netherlands
Year
1991
Tongue
English
Weight
776 KB
Volume
116
Category
Article
ISSN
0301-486X

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✦ Synopsis

A new minimal synthetic medium, with low amount of glucose, without aminoacids, vitamins and neutral pH, which induces germ-tubes production in Candida albicans, is reported in this work. The results indicate a perfect agreement between the germ-tube test performe d with the standard method in human or animal serum and this test performed in minimal synthetic medium. In this medium the germ-tube test for the presumptive identification of Candida albicans can be performed with the same formality, time and reproducibility as those in human or animal serum. This constitutes an interesting finding because it is easy to prepare, to store and is highly reproducible.

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Optimal concentration of ammonium ion in
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✍ Valerio Vidotto; Laura Guevara Ochoa; Juan Michea Cortes; Massimo Bruatto πŸ“‚ Article πŸ“… 1991 πŸ› Springer Netherlands 🌐 English βš– 358 KB

Candida albicans strain B 311-10 with and without starvation was cultivated in the minimal synthetic medium of Shepherd et al. [ 18], modified without biotin, aminoacids, low glucose concentration [20] and with decreasing amounts of (NH4) 2 S 04, to determine the optimal growth requirement for this