Optimal concentration of ammonium ion in a minimal synthetic medium for the growth ofCandida albicans
✍ Scribed by Valerio Vidotto; Laura Guevara Ochoa; Juan Michea Cortes; Massimo Bruatto
- Book ID
- 104772386
- Publisher
- Springer Netherlands
- Year
- 1991
- Tongue
- English
- Weight
- 358 KB
- Volume
- 113
- Category
- Article
- ISSN
- 0301-486X
No coin nor oath required. For personal study only.
✦ Synopsis
Candida albicans strain B 311-10 with and without starvation was cultivated in the minimal synthetic medium of Shepherd et al. [ 18], modified without biotin, aminoacids, low glucose concentration [20] and with decreasing amounts of (NH4) 2 S 04, to determine the optimal growth requirement for this strain. All the experiments were carried out under sterile conditions at 25 ~ in a thermostat with initial O.D.s (675 nm) of 0.500 and 0.100. Cell growth was generally monitored everyday for six days with a spectrophotometer by determining the absorbance of the cultures at 675 nm. All the experiments were repeated three times and a statistical analysis of the data with a probability of 99 ~o and 1 ~o of error was performed to confirm the validity of the results. Best growth was obtained with starved cells at an initial O.D. of 0.100 and with a 0.1 g/L concentration of (NH4)2SO 4 . At this concentration, the growth of C. albicans B 311-10 was best between the first and the fourth day with the maximum at the third day. With (NH4)2SO 4 concentrations of 0.05 and 0.5 g/L, cell growth was the same.