๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A new method for the rapid separation of cell organelles

โœ Scribed by Heinz Detlef Gregor

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
165 KB
Volume
82
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

A rapid procedure is described for the separation of plant cell organelles from castor bean endosperm (Ricinus communis).

This method is based on the reorientation of sucrose density gradients during centrifugation in a vertical rotor, thus resulting in a shorter path length and drastically reduced run times. Comparison to a separation by a standard procedure shows that, by using this method, equal resolution is possible in less than 10% spin time.

๐Ÿ“œ SIMILAR VOLUMES

A rapid method for separation and assay
A rapid method for separation and assay of radiolabeled mucopolysaccharides from cell culture medium
โœ Heikki Saarni; Markku Tammi ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 308 KB

A method is described for the separation and assay of radiolabeled glycosaminoglycuronans (GAGS) from cell culture medium. Following papain digestion and precipitation with cetylpyridinium chloride onto cellulose ester filter membrane, hyaluronic acid can be separated from sulfated GAGS by selective

New rapid flow cytometric method for the
New rapid flow cytometric method for the enumeration of nucleated red blood cells
โœ Tomohiro Tsuji; Takashi Sakata; Yukio Hamaguchi; Fu-sheng Wang;; Berend Houwen ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 512 KB

Background: Nucleated red blood cells (NRBC) in blood specimens compromise the automated white blood cell (WBC) count on most hematology analyzers. This makes it necessary to correct the WBC count by subtracting separately counted NRBC by manual microscopy. In addition, it is clinically important to

A new, convenient method for the rapid a
A new, convenient method for the rapid analysis of inorganic pyrophosphate
โœ Harold L. Drake; Neil H. Goss; Harland G. Wood ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 301 KB

A new method for the rapid analysis of inorganic pyrophosphate (PPJ which utilizes the enzyme ATP sulfurylase is described. All components of the assay system are commercially available and inexpensive. The assay is linear over the range of 0.5-50.0 nmol of PP, and is not affected by inorganic phosp