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A new method for precipitating bacterial exopolysaccharides

✍ Scribed by Joana Azeredo; Rosário Oliveira

Publisher: Springer-Verlag
Year: 1996
Tongue: English
Weight: 276 KB
Volume: 10
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00173251

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✦ Synopsis

Several methods for exopolysaccharide precipitation are compared and a new method is proposed which uses nitron (3,5,6-triphenyl-2,3,5,6-tetraaza[2.1 .l]bicyclo-1-hexene) as the precipitating agent and gives recoveries of about 99%, using only 0.1 vol.. The highest recovery obtained with the usual methods was around 75%, using 3 vol. propanol, but this makes the final recovery more difficult.

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