A new method for immobilization of acetylcholinesterase
✍ Scribed by Hayrettin Tümtürk; Ferat Şahin; Gökhan Demirel
- Book ID
- 106146708
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 218 KB
- Volume
- 30
- Category
- Article
- ISSN
- 1615-7605
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Polygalacturonase and pectinesterase have been successfully immobilized on gamma alumina by activation of the support with glutaraldehyde at pH 3.0. The half life of the enzymes increased by four and two fold compared to the immobilization on gamma alumina without activation.
Different methods for the immobilization of acetylcholinesterase (AChE), viz, co-cross-linking at the surface of a pH electrode, co-cross-linking in a membrane and trapping in a polyacrylamide membrane, are described The immobilization products are compared with respect to the response times, the re
Acetylcholinesterase from electric eel is selectively immobilized on Amberlite IR-120 resin equilibrated with Al 3+ ions. Immobilized acetylcholinesterase activity is stable at least for 85 days in the wet state at 10°C and for 180 days in the dry state at room temperature. Activity determinations i