The periplasmic phosphate binding protein is a product of the phoSgene and is an essential component of the phosphate specific transport (PST) system, which mediates Pi uptake in Escherichia coll. The binding of Pi to periplasmic protein(s) and the kinetic parameters of Pi uptake were studied in pho
A new locus in the phosphate specific transport (PST) region of Escherichia coli
β Scribed by Levitz, Ruth ;Klar, Avihou ;Sar, Nehemia ;Yagil, Ezra
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 555 KB
- Volume
- 197
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
PhoS64 is a mutation in the Phosphate Specific Transport (PST) region on the E. coli chromosome which lacks the periplasmic phosphate binding protein. In contrast to other phoS mutations (which have the same phenotype) it complements the mutations in phoT and pstB. A detailed genetic map of the PST region constructed by three point transductional crosses has revealed that phoS64 is located distally from other phoS mutations. The genetic order obtained was phoS64-phoU35-pstB401-phoT-phoS-ilvC. The data indicate that phoS64 belongs to a different complementation unit in the PST region not known hitherto. We propose to name it phoV.
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