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A new interferometric technique for the determination of the electrophoretic mobility of macromolecules

✍ Scribed by William F. Pickard; Krishan C. Sehgal

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 695 KB
Volume: 96
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90593-1

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✦ Synopsis

A novel device is described for measuring the electrophoretic mobility of monodisperse macromolecules. This apparatus, which is based upon a Twyman-Green interferometer, yields 43% precision with runs lasting only 15 min; and it is suitable for temperatures and/or ionic strengths near or below physiological. Its operation is illustrated with bovine serum albumin, the apparent mobility of which is shown to vary strongly with the purity and concentration of the preparation.

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