Capillary zone electrophoresis for the determination of electrophoretic mobilities and diffusion coefficients of proteins

✍ Scribed by Yvonne Walbroehl; James W. Jorgenson

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 481 KB
• Volume: 1
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.1220010110

✦ Synopsis

Capillary zone electrophoresis can be used to determine mobilities and diffusion coefficients for analytes. Mobilities are determined from measurements of migration times. Correction for electroosmotic flow must be made by measuring the flow magnitude using an uncharged marker substance. Diffusion coefficients can be measured from peak widths of analyte bands. Care must be taken in these measurements that the analyte concentration does not overload the system and perturb the axial homogeneity of the electric field strength. Analyte also must not adsorb to the surface of the capillary. Finally, the capillary should be operated with a power dissipation that is low enough that minimal heating of the capillary occurs. Capillary zone electrophoresis offers some advantages over other methods of determining mobilities and diffusion coefficients. These include a requirement of only small quantities of analyte at relatively low concentrations and the capability of making measurements on several substances simultaneously in mixtures.