A new gene controlling the frequency of cell division per round of DNA replication in Escherichia coli
β Scribed by Nishimura, Akiko
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 889 KB
- Volume
- 215
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Several conditional lethal mutants of Escherichia coli have been analysed genetically using generalized transduction and lambda transducing vectors. Three temperature-sensitive ftsE mutants were found as was a cold-sensitive ftsE mutant. A new gene was found which mapped close to ftsE, namely ftsS.
We have constructed a pBR322 plasmid derivative which expresses dnaA protein under the control of the E. coli lac UV5 promotor. Expression of the dnaA protein from the plasmid is inducible by isopropyl-beta-D-thiogalactoside. In a dnaA+ strain induction has no effect on the accumulation of DNA. In c