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Increased expression of the dnaA gene has no effect on DNA replication in a dnaA + strain of Escherichia coli

✍ Scribed by Churchward, G. ;Holmans, P. ;Bremer, H.

Publisher: Springer
Year: 1983
Tongue: English
Weight: 344 KB
Volume: 192
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00392197

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✦ Synopsis

We have constructed a pBR322 plasmid derivative which expresses dnaA protein under the control of the E. coli lac UV5 promotor. Expression of the dnaA protein from the plasmid is inducible by isopropyl-beta-D-thiogalactoside. In a dnaA+ strain induction has no effect on the accumulation of DNA. In contrast, in a thermosensitive dnaA46 strain, induction, at either the permissive or the nonpermissive temperature, results in an immediate stimulation of DNA accumulation. We conclude that, while in a dnaA46 strain dnaA protein limits DNA replication, in a dnaA+ strain dnaA protein activity does not control the timing of replication initiation.

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