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A new enzymatic route to the synthesis of 12-ketoursodeoxycholic acid

โœ Scribed by Roberto Bovara; Giacomo Carrea; Sergio Riva; Francesco Secundo

Book ID
104640004
Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
287 KB
Volume
18
Category
Article
ISSN
0141-5492

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โœฆ Synopsis

Cholic acid (3a.7a12a-trihydroxy-5~cholanoic acid) was completely and selectively transformed into 1Zketomsodeoxycholic acid (3a,7@lihydroxy-12-oxo-Sf&cholanoic acid) by means of hvo consecutive enzymatic steps catalyzed, the fifit. by 7u-and 12a-hydroxysteroid dehydrogenase and, the second, by 76hydroxysteroid dehydrogenase. Coenzyme regeneration was carried out with a-ketoglutarate-glutamate dehydrogenase and glucose-glucose dehydrogenase, respectively.

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## Abstract magnified image The hydroxysteroid dehydrogenases (HSDHs)โ€catalyzed oneโ€pot enzymatic synthesis of 12โ€ketoursodeoxycholic acid (3ฮฑ,7ฮฒโ€dihydroxyโ€12โ€oxoโ€5ฮฒโ€cholanoic acid), a key intermediate for the synthesis of ursodeoxycholic acid, from cholic acid has been investigated. This goal has