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A new enzymatic cycling method for ammonia assay using NAD synthetase

✍ Scribed by Fumihiko Yamaguchi; Takashi Etoh; Mamoru Takahashi; Hideo Misaki; Haruhiko Sakuraba; Toshihisa Ohshima

Book ID
116350013
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
452 KB
Volume
352
Category
Article
ISSN
0009-8981

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A rapid enzymatic assay method for ammonia was developed by using glutamine synthetase from glutamate-producing bacteria together with pyruvate kinase, lactate dehydrogenase, and NADH. The time required for determination of 25 nmol of ammonia was 5 min with 1 unit of glutamine synthetase, as opposed