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A colorimetric method using chlorpromazine-5-oxide for the assay of NAD- and NADP-oxidoreductases

โœ Scribed by Lee, Kum-Tatt ;Seet, Ai-Mee


Book ID
105113344
Publisher
Springer-Verlag
Year
1978
Weight
599 KB
Volume
70
Category
Article
ISSN
0344-838X

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Amplified colorimetric assay of alkaline
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Alkaline phosphatase hydrolyzes riboflavin 4'-phosphate to produce riboflavin. This is converted to riboflavin 5'-phosphate, using riboflavin kinase, which reconstitutes apoglycolate oxidase to give hologlycolate oxidase. This enzyme catalyzes the oxidation of glycolate with simultaneous production