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A new conditional lethal mutator (dnaQ49) in Escherichia coli K12

โœ Scribed by Horiuchi, Takashi ;Maki, Hisaji ;Sekiguchi, Mutsuo

Publisher
Springer
Year
1978
Tongue
English
Weight
652 KB
Volume
163
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

A conditional lethal mutator, dnaQ49, was found in Escherichia coli K12. The dnaQ49 mutation caused stimulation of rifampicin-, nalidixic acid- and streptomycin-resistant mutation frequencies 100 to 2000 fold at 30 degrees C and the frequencies were further increased 50 to 100 fold at 35 degrees C or higher temperatures. Cells carrying dnaQ49 were unable to grow in salt-free L-broth at 44.5 degrees C, and DNA synthesis but not protein synthesis of the cells was suppressed under the restrictive conditions. The dnaQ gene was located at about 5 min on the E. coli linkage map and the order of the genes residing in this region was determined to be ton A-dnaE-metD-dnaQ-pro A.

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