โ Scribed by M.Renuka Prasad; M. Sugumaran; C.S. Vaidyanathan
No coin nor oath required. For personal study only.
A new, rapid, simple, and sensitive calorimetric method for the estimation of resorcinol in microgram amounts is described.
Rothera's test (1) has been routinely used to identify the presence of active -CH,-CO-CH,-groups in various biological samples. Thus, the formation of 3-oxoadipic acid from protocatechuic acid (2) and pyrocatechuic acid (3) has been demonstrated qualitatively by the application of this test. During our studies on the metabolism of resorcinol by microorganisms, we observed the formation of a green-colored complex when Rothera's test was conducted on a reaction mixture containing resorcinol. When this color test was used in an attempt to estimate resorcinol quantitatively, it was noted that the presence of hydroxylamine hydrochloride caused a considerable increase in the intensity of the color. By making use of these findings and after a detailed study of the factors affecting the color production, a rapid, simple, and sensitive method was developed for the estimation of resorcinol.
A method for the separation of resorcinol from interfering substances by paper chromatography is also presented.
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