A new assay for the analysis of X-chromosome inactivation in carriers with an X-linked disease
β Scribed by Takeo Kubota
- Book ID
- 117545572
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 238 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0387-7604
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High resolution chromosome analysis was done on lymphoblastoid cell lines, established during the past decade from affected males with X-\inked lymphoproliferative disease (XLP) or from obligate female carriers, from 14 families. One cell line, from a male with XLP, has a partial deletion of band Xq
The pattern of X-chromosome inactivation was analyzed, by means of two different DNA probes (pSPT-PGK and M27 beta), in several cell lineages derived from females belonging to a pedigree with X-linked immunodeficiency with hyper-IgM (HIGM1). Non-random X-chromosome inactivation was demonstrated in T