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A new approach to isolation and culture of human Kupffer cells

✍ Scribed by Edward B. Alabraba; Stuart M. Curbishley; Wai K. Lai; Stephen J. Wigmore; David H. Adams; Simon C. Afford


Book ID
113717413
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
378 KB
Volume
326
Category
Article
ISSN
0022-1759

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## Abstract We report a four‐step procedure that optimizes the methodology for isolation of highly purified rat Kupffer cells (KC). We combined the previously reported techniques of enzymatic tissue treatment, density gradient centrifugation, centrifugal elutriation and selective adherence. ED‐2 im