𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A new approach to biofunctionalisation and micropatterning of multi-well plates

✍ Scribed by Kristina Lehmann; Manuela Herklotz; Martin Espig; Tina Paumer; Mirko Nitschke; Carsten Werner; Tilo Pompe

Book ID
104004018
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
970 KB
Volume
31
Category
Article
ISSN
0142-9612

No coin nor oath required. For personal study only.

✦ Synopsis

Biomolecule attachment and lateral micropatterning of biomolecular layers are essential techniques to provide in advanced biochemical and cell culture assays. For that purpose, we introduced a versatile, simple and robust method to functionalise standard polystyrene well plates. Free amino groups were generated on the polystyrene surface by low pressure ammonia plasma treatment. Subsequently, thin films of different maleic anhydride copolymers were covalently attached to the surfaces. The distinct physicochemical properties of the coupled maleic anhydride copolymers provided a broad range of possible attachment schemes of proteins and polysaccharides. Micrometer-sized lateral patterns of these functional coatings were created by plasma etching through silicon masks and subsequent chemical conversion of the etched areas using poly(ethylene glycol). The approach facilitates a wide variety of cell culture experiments allowing a combination of biomolecule coupling and micropatterning within the multi-well plate technology.

πŸ“œ SIMILAR VOLUMES

A new approach to the design of internal
A new approach to the design of internal fixation plates
✍ Woo, Savio L-Y. ;Simon, Bruce R. ;Akeson, Wayne H. ;Gomez, Mark A. ;Seguchi, Yas πŸ“‚ Article πŸ“… 1983 πŸ› John Wiley and Sons 🌐 English βš– 749 KB

Mathematical analysis by finite element modeling methods was used in conjunction with laboratory bench experiments in selecting appropriate stiffness parameters for internal fixation plate designs. Bench experiments performed included tests of an idealized plated magnesium tube and of a plated canin

A new approach to the forced vibration o
A new approach to the forced vibration of thin plates
✍ B.K. Donaldson πŸ“‚ Article πŸ“… 1973 πŸ› Elsevier Science 🌐 English βš– 932 KB

This paper presents a new, approximate method of analysis in the classical style for the response of a thin plate to a single frequency, forced harmonic vibration. The plate may be quadrilateral or triangular, and it may have any boundary conditions. The distinctive feature of the method is the mani