A New Approach for the Detection and Identification of Protein Impurities Using Combinatorial Solid Phase Ligand Libraries
✍ Scribed by Fortis, Fréderic; Guerrier, Luc; Areces, Liliana B.; Antonioli, Paolo; Hayes, Tim; Carrick, Kevin; Hammond, David; Boschetti, Egisto; Righetti, Pier Giorgio
- Book ID
- 126205375
- Publisher
- American Chemical Society
- Year
- 2006
- Tongue
- English
- Weight
- 313 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1535-3893
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📜 SIMILAR VOLUMES
## Abstract A new methodology for the rapid assessment of affinity ligands synthesized by combinatorial solid‐phase chemistry is reported. This screening strategy utilizes the target protein conjugated to FITC, and represents an almost universal technique for the preliminary screening of solid‐phas
HPLC was used in combination with immuno-bead separation technique for identification of an individual protein from a pool of proteins. This was carried out using an in-house monoclonal antibody (ATC2) specific for placental alkaline phosphatase (PLAP) as a primary antibody for conjugation to CNBr b