A new approach for chemical phosphorylation of oligonucleotides at the 5′-terminus
✍ Scribed by Andrei Guzaev; Harri Salo; Alex Azhayev; Harri Lönnberg
- Book ID
- 103399593
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- French
- Weight
- 727 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0040-4020
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📜 SIMILAR VOLUMES
Surmaary: Reaction of the 5' hydroxyl group of a support bound synthetic oligonucleotide with the new reagent N-trifluoroacetyl-2-aminoethyl-(2-cyanoethyl)-N,N-diisopropylaminophosphoramidite yields, upon deprotection, an oligonucleotide having a 5' primary amino terminus. The amino derivatized olig
A lipophilic phosphorylating agent was prepared and used for the synthesis of pentadeoxyribonucleotide with aminoethyl group at 5' end on a polymer support by the phosphotriester method. Chemically synthesized oligodeoxyribonucleotide with defined sequence is widely used as the primer for DNA sequen