✍ Scribed by Lei Ye; Olof Ramström; Mats-Olle Månsson; Klaus Mosbach
No coin nor oath required. For personal study only.
We have studied the possibility of shifting a thermodynamically unfavourable enzymatic equilibrium towards product formation via the addition of a highly specific adsorbent. The commercially interesting enzymatic condensation of Z-L-aspartic acid with L-phenylalanine methyl ester to the sweetener aspartame was chosen as the model system. Extremely stable and specific adsorbents for the product Z-L-Asp-L-Phe-OMe (Z-aspartame) were prepared using the emerging technique of molecular imprinting. A considerable increase (40%) in the yield of product was obtained when such adsorbents were present during the enzymatic reaction. The message of this investigation is that the use of such specific, sterilizable adsorbents should be considered for enzymatic processes to increase the yield. Finally, the direct isolation of a product formed by the retrieval of the adsorbents carrying the product can be envisaged, especially if the adsorbents are magnetic.
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Biomimetic testosterone receptors were synthesized via molecular imprinting for use as antibody mimics in immunoassays. As evaluated by radioligand binding assays, imprinted polymers prepared in acetonitrile were very specific for testosterone because the nonimprinted control polymers bound virtuall
Schematic representation of molecular imprinting of the transition state analogue 2 in the polymer matrix.
2-Hydroxy-ethyl-methacrylate-based molecularly imprinted polymer imprinted for hydrocortisone was found to absorb a considerable amount of testosterone. The release of testosterone to water was found to be very slow. Its release was, however, rapid in the presence of the print molecule (hydrocortiso