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A multisample chamber for dehydration and critical point drying

✍ Scribed by Gollin, Susanne M. ;Wray, Wayne

Publisher: Wiley (John Wiley & Sons)
Year: 1984
Tongue: English
Weight: 827 KB
Volume: 1
Category: Article
ISSN: 0741-0581
DOI: 10.1002/jemt.1060010208

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✦ Synopsis

The principles and methods for constructing an improved chamber for dehydration and critical point drying of multiple biological samples are described. The specimen chamber design is based on vertical positioning of the electron microscope grids or coverslips and permits minimal perturbation of laminar solvent flow past the specimens. This condition is requisite for optimal exposure of samples to solvents, which is necessary for complete dehydration and drying. Fragile samples, including chromosomes, critical point dried in the multisample chamber demonstrate crisp, well-preserved, three-dimensional morphology.

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