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A Multiplexed Protein Kinase Assay

โœ Scribed by Melissa D. Shults; Igor A. Kozlov; Nicholas Nelson; Bahram G. Kermani; Peter C. Melnyk; Veronika Shevchenko; Anu Srinivasan; Joseph Musmacker; John P. Hachmann; David L. Barker; Michal Lebl; Chanfeng Zhao

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
409 KB
Volume
8
Category
Article
ISSN
1439-4227

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๐Ÿ“œ SIMILAR VOLUMES

A rapid paper chromatographic assay for
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SHORT COMMUNICATIONS ## A Rapid Paper Chrokatographic Assay for Protein Kinase This communication describes a simple, rapid, and effective paper chromatographic method for separating the trichloroacetic acid (TCA) insoluble phosphorylated histone from its substrate Y-~~P-ATP in the protein kinas

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Protein kinase (EC 2.7.1.37) catalyzes the phosphorylation of serine and threonine residues of a number of proteins. Histone is widely used as an acceptor substrate in measuring the activity of this enzyme isolated from a variety of sources. We have devised a rapid procedure for resolving phosphohis