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A multiple-column approach to the methylation analysis of plant cell-walls

✍ Scribed by James A. Lomax; Alex H. Gordon; Andrew Chesson

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
761 KB
Volume
138
Category
Article
ISSN
0008-6215

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✦ Synopsis

A procedure has been developed for the improved analysis of the neutralsugar glycosidic linkages in plant cell-walls, utilising capillary g.1.c. and columns of three different phases for the separation of the products of methylation analysis. Retention coefficients are reported for a wide range of partially methylated alditol acetates on columns of CP-Si188 and a bonded phase (BP-l) equivalent to OV-1. Using these phases and SP-1000, all cases of co-chromatography can be resolved. Computers were used to process the large amounts of data produced, to identify peaks and to assist in merging the results obtained using the three phases.

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