A monoclonal antibody recognizing HLA-DR2 on malignant and activated cells

✍ Scribed by Susan V. Fuggle; Jean Kirkley; Alan Ting; Peter J. Morris

• Book ID: 104746007
• Publisher: Springer-Verlag
• Year: 1983
• Tongue: English
• Weight: 978 KB
• Volume: 18
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00372473

✦ Synopsis

A monoclonal antibody, designated NDS15.38, which recognizes a polymorphic determinant of HLA-DR, was produced from a fusion in which mice were immunized with the human B lymphoblastoid cell line GIR2 (HLA type A1, B8, 27, Cw2, DR2,7). NDS15.38 functions efficiently as an affinity column and purifies a two-chain complex of molecular weight 33 000 and 30 000 under reducing conditions. The monoclonal antibody reacts with HLA-DR2-positive B lymphoblastoid cell lines and B lymphocytes from patients with chronic lymphatic leukemia in an indirect radioactive binding assay. However, NDS15.38 does not appear to react with peripheral blood B lymphocytes from normal individuals. Using a peroxidase staining technique, NDS15.38 was shown to react with phytohemagglutin (PHA)-stimulated lymphocytes and with apparently activated B cells in the germinal centers of lymph nodes from individuals who were tissue typed as HLA-DR2. Thus it appears that NDS15.38 recognizes a polymorphic determinant of HLA-DR on malignant and stimulated cells, but not on resting cells.