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A molecular analysis of terminase cuts in headful packaging of Salmonella phage P22

✍ Scribed by Schmieger, Horst ;Taleghani, KambizMansouri ;Meierl, Anita ;Wei�, Ludwig

Publisher
Springer
Year
1990
Tongue
English
Weight
430 KB
Volume
221
Category
Article
ISSN
0026-8925

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✦ Synopsis

Fragments of DNA molecules of Salmonella phage 22 which represent the molecular termini created by the terminase reaction have been cloned and sequenced. The terminase cleavage separates a headful-sized piece of DNA from the concatemeric precursor; by successful cloning strategy it was shown that the terminase produces blunt ends. The termini of 20 different phage DNA molecules fall into a region located between about 600 and 4000 bp from the pac signal and show a Gaussian distribution. The average terminal redundancy was calculated to be about 2230 bp (= 5.3%) and is therefore higher than was previously reported. A comparison of the nucleotides flanking the terminal bases of 20 different end clones does not support the suggestion that the terminase recognizes some specific sequence and/or structural information in determining the actual cleavage site.

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