The origin of the DNA in a special class of generalized transducing particles of Salmonella-phage P22
โ Scribed by Backhaus, Horst ;Schmieger, Horst
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 714 KB
- Volume
- 131
- Category
- Article
- ISSN
- 0026-8925
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Lysates of P22 contain a small fraction of transducing particles with bacterial DNA replicated semiconservatively after the time of infection. It was demonstrated that the presence and relative amount of this class of transducing particles was unchanged, if infection of Salmonella occured under a co
The origin of DNA in transducing particles of bacteriophage Mu was investigated by density labelling techniques. Unlabelled plaque-forming and leu+-transducing particles were of about the same density. Preinfection labelling of DNA with 5-bromodeoxyuridine increased the density of the transducing pa
The kinetics of production of transducing particles for different bacterial markers were followed by premature lysis of Salmonella typhimurium cells infected with P22 phages. The were compared for cells infected with wild type phage or with HT-mutants which show increased transduction frequencies. M